Mesotherapy for Alopecia


Mesotherapy was originated by Michel Pistor, a French doctor in 1952. Meso means ‘middle’ and the concept of mesotherapy is medical technique of mesodermic treatment.
It is a technique field of experience, so it has been developed for doctors that they could demonstrate the long-time skilled ability and technique of their own.
Bonnet Huteau explained the action of mesotherapy with 4unit theory of Fundamental Competence Unit, Microcirculatory competence unit, Neurovegetative competence unit, Immune competence unit.
A. Kaplan explained mesotherapy functions with the pharmaceutical action of receptors around local area existing considerable period and effectiveness of long distance by chemical transmission.
Aesthetic mesotherapy is applied to various field of Alopecia, Anti-Aging, Lifting & glow, Anti-melasma, Localized Adiposity, Cellulite. And alopecia is the best use of mesotherapy field.
But mesotherapy has the risk of pain, allergy, inflammation, or necrosis by unskilled technique or inappropriate pharmacology.
So mesotherapy needs enough experienced skill on basic principles.

1. Mesotherapy techniques

① Method of meso-solution delivery
– Manual injection
– Meso-gun: Injecting device controlled by electric motor or air compressor
– MTS (Micro-needling therapy system): Meso-roller, meso-stamp, Auto-MTS

② Depth of procedure
– IED (Intra-epidermic)
  Epidermal target, no bleeding, painless, Play for keratinization stimulation
– IDS (Intradermal superficial)
  Basal layer & Papillary dermis target, small pain, Play for stimulation of vascular networks and stem cell area. Main target of alopecia solutions.
– IDP (Intradermal propound)
  Reticular dermis target, a little painful, Stimulation of dense irregular connective tissue. Play for vasodilator delivery and PRP.

③ Injection technique
– P.P.P. (Point par Point):
  Intra dermal profound target
  Depth (4~10mm), Vol. (0.3~0.4ml)
  Sever pain control, Injection for trigger points
– Papule
  Between epidermis and superficial dermis (basal layer) target
  Depth (1~2mm), Vol. (0.1ml> )
  Transient storage in upper dermis skin
– Nappage
  Multi-injection with IED or IDS/IDP
  Inject 2~4 times every second with 2~4mm interval
  Spreading injection technique in speed

④ Dry & Wet
– Dry therapy
  Mechanical stimulation by dry needle. Not use any drug
– Wet therapy
  After applying aesthetic solution on the skin, stimulate the skin with needle.
  MTS therapy

⑤ Kaplan’s Meso interface

Injection(1cc) Point number Point Volume Meso Interface(㎠)
Injection(P.P.P) 3 0.33 2.3
5 0.20 4.8
10 0.10 10.00
Nappage 100 0.01 22.4
150 0.006 25.7
200 0.005 28.2

2. Mesotherapy principles

① Basic principle
– Multiple injection technique with fine needle(30G)
– Small does, rarely, and right points
– Formulation: Less than 3agents(Cf. 5Agent & Dilution)
– Safety & efficacy approved multi solution (ex. AGF39™)
– Agents for local effect
– Well accustomed to skin biology

② Pharmacology Principle
– The most important principle is experience
– Isotonicity
– Integration with the tissue of injected substances
– Synergistic
– Physical, Chemical compatibility (ex. Color, pH, solubility)
– Non therapeutic antagonism
– Formulation: Less than 3agents(Cf. 5Agent & Dilution)
– Safety & efficacy approved multi-nutrients(AGF39™)

③ Guidance for drug selection
– Isotonicity
– Tolerance of skin or subcutaneous tissue
– Absence of pain, nodule formation, or necrosis
– Integration with the tissue of injected substances
– Absence of possible incompatibilities
– Avoidance of allergies or hypersensitivity
– Non-use of oily solution
– Efficacy verified

3. Mesopecia(Mesotherapy for Alopecia)

① Indication
– Androgenetic Alopecia (N-H type I~IV)
– Women’s Alopecia (L. type I~II)
– Chronic Telogen E.

② Procedure objectives
– Normalization of hair cycle
– Rebalancing of cell cytokines of follicle
– Nourishment (ECM stimulation)
– Stimulation of circulation
– Stimulation of follicle cell vitality
– Stimulation of follicle keratinocyte
– Stimulation of anchoring molecules production
– Improvement of immunity (AA)
– Inhibition of DHT
– Anti-free radicals & inflammation

③ Interval of Mesopecia
– 2-week interval is basic process
– Recommended process in actual.
  • Start every week term for 5 ~ 10 times
  • Develop every two-week term on efficacy response
  • Develop every three-week term on efficacy progress
  • Maintain monthly care and equivalent change on efficacy response

4. Mesotherapy for AA

① Triamcinolone injection in the local point of symptom
– The main treatment for AA. Pain control is needed for pediatric patients.

② Others: Cryotherapy, ultraviolet light therapy, DPCP, immunodepressant etc.

5. Recommendable ingredients for Mesopecia

The most important ingredient should regulate and normalize the mechanism of hair growth.

① AGF39™
The methods for obtaining the cytokines for hair treatment are Recombination (AGF39™), Stem cell media extraction (Exosome), PRP (Platelet rich plasma)
Patients can meet the products made of cytokines for hair growth and inhibiting cytokines against the hair loss inducing cytokines is real by up-to-date technology of AGF39™.

② Others
Taurine, Gingko biloba, Organic silicium, Centella asiatica, Biotin, Dexpanthenol, HA, Finasteride (developing), Minoxidil (developing), Calcitonine, Placenta extract, Triamcinolone acetonide, Polyvitamin

6. CytoMesopecia

The life of cell of differentiation, growth, migration, and death is all controlled by cytokines which are synthesis and secreted from cell themselves and neighborhood cells. At the same way, hair loss is occurred by cell apoptotic cytokines synthesized by DHT and derived from around dermal papilla.
The most common follicle cell apoptotic cytokines are TGF-β2, TGF-β1, DKK-1, BMP, IL-1, FGF5, TNF-α etc. which cytokines induce protein degradation and increase cell apoptotic factors.
Recent studies have released that using efficacious cytokines (FGF9, Noggin, SOD) which are the antagonist of apoptotic cytokines can inhibit the apoptosis inducing cytokines without any serious systemic influence to the body. With this way, we can regulate hair cycle against DHT induced hair loss.
Furthermore, the additional use of the growth cytokines like KGF, bFGF, IGF-1, VEGF etc. increases hair cell proliferation and activates the essential Wnt/β-catenin signal then promotes hair regrowth and maintains anagen phase.
Now we can make hair cell growing cytokines such as IGF-1, VEGF, bFGF, KGF and anti-cytokines against cell apoptotic cytokines such as Noggin, FGF9 and Super oxide dismutase.
CytoMesopecia is to keep cytokine balance in the face of DHT induced cell apoptotic cytokines.
AGF39™ is a proven Mesopecia formula with 3 anti-apoptotic cytokines and 4 cell growing cytokines by many clinical tests including 1 SCI degree journal and 2 WCHR revealed study.